ZNF24 Polyclonal antibody

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• ZNF24 Polyclonal antibody (11219-1-AP)

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• KD/KO Validated

ZNF24 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11219-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

Zinc finger protein 191, Zinc finger and SCAN domain-containing protein 3, Zfp191, RSG-A, RSG A

• 

  WB analysis of HeLa using 11219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 11219-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 11219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11219-1-AP

  Immunofluorescent analysis of HeLa cells using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11219-1-AP targets ZNF24 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1724

  Product name: Recombinant human ZNF24 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-368 aa of BC016801

  Sequence: MSAQSVEEDSILIIPTPDEEEKILRVKLEEDPDGEEGSSIPWNHLPDPEIFRQRFRQFGYQDSPGPREAVSQLRELCRLWLRPETHTKEQILELVVLEQFVAILPKELQTWVRDHHPENGEEAVTVLEDLESELDDPGQPVSLRRRKREVLVEDMVSQEEAQGLPSSELDAVENQLKWASWELHSLRHCDDDGRTENGALAPKQELPSALESHEVPGTLSMGVPQIFKYGETCFPKGRFERKRNPSRKKQHICDECGKHFSQGSALILHQRIHSGEKPYGCVECGKAFSRSSILVQHQRVHTGEKPYKCLECGKAFSQNSGLINHQRIHTGEKPYECVQCGKSYSQSSNLFRHQRRHNAEKLLNVVKV

• Full Name: zinc finger protein 24
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC016801
• Gene Symbol: ZNF24
• Gene ID (NCBI): 7572
• RRID: AB_10888626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17028
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 24 (ZNF24; also known as KOX17, Zfp191 or ZNF191) is a member of the Kruppel-like zinc finger (ZF) transcription factor family, which plays a critical role in cell proliferation and differentiation. There're two isoforms with calculated MW 42KDa and 22KDa. ZNF24 protein can be modificated with SUMO.

Publications

What published studies show

The ZNF24 antibody (Cat# 11219-1-AP) has 7 total citations. These appear in journals including Redox Biology, Cell Death & Disease, Oncogene, BMC Cancer, Experimental Cell Research, and Frontiers in Oncology. The associated research fields span cancer biology—covering renal cell carcinoma, pancreatic cancer, bladder cancer, lung adenocarcinoma, and colorectal cancer—as well as immunometabolism and transcription factor regulation. Applications cited include WB, IHC, IP, IF, and ChIP.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Redox Biol; Epithelial redox stress programs macrophage immunometabolism through a ZNF24-MIF-NF-κB pathway in chronic nonbacterial prostatitis.; Authors - Fei Zhang ; KD Validated
human	WB,IP	Cell Death Dis; The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma; Authors - Ji Liu
human	WB,IHC	Cell Death Dis; LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway.; Authors - Wei Li ; KD Validated
mouse	IHC	Oncogene; Crosstalk between SUMOylation and ubiquitination controls the stability of transcription factor zinc finger protein 24: a novel antitumor mechanism in bladder cancer; Authors - Xiaosong Wei
human	WB,IHC	BMC Cancer; KRAS mutation promotes immune escape of lung adenocarcinoma via ZNF24/SLC7A5/PD-L1 axis.; Authors - Leilei Li ; KD Validated
human	IHC	Exp Cell Res; Tumor-suppressive zinc finger protein 24 (ZNF24) sensitizes colorectal cancer cells to 5-fluorouracil by inhibiting the Wnt pathway and activating the p53 signaling; Authors - Fanqi Meng