ZNF263 Polyclonal antibody

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  IP experiment of HepG2 using 21326-1-AP

  IP result of anti-ZNF263 (IP:21326-1-AP, 4ug; Detection:21326-1-AP 1:300) with HepG2 cells lysate 4000ug.

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  IF Staining of U2OS using 21326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF263 antibody (21326-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

ZNF263 Polyclonal Antibody for IF/ICC, IP, ELISA

Cat No. 21326-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ChIP, ELISA

Synonyms

ZKSCAN12, Zinc finger protein FPM315, Zinc finger protein 263, ZFP263, FPM315

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Tested Applications

• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 2 publications below

Product Information

21326-1-AP targets ZNF263 in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15158

  Product name: Recombinant human ZNF263 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 334-599 aa of BC008805

  Sequence: LGRPHDRSQGDWAPPPEGGMEQALAGASSGRELGRPKELQPKKLHLCPLCGKNFSNNSNLIRHQRIHAAERLCMGVDCTEIFGGNPRFLSLHRAHLGEEAHKCLECGKCFSQNTHLTRHQRTHTGEKPYQCNICGKCFSCNSNLHRHQRTHTGEKPYKCPECGEIFAHSSNLLRHQRIHTGERPYKCPECGKSFSRSSHLVIHERTHERERLYPFSECGEAVSDSTPFLTNHGAHKAEKKLFECLTCGKSFRQGMHLTRHQRTHTG

• Full Name: zinc finger protein 263
• Calculated Molecular Weight: 683 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC008805
• Gene Symbol: ZNF263
• Gene ID (NCBI): 10127
• RRID: AB_2878841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14978
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF263 is a transcriptional repressor and activator that contains an N-terminal scanning domain and nine C2H2 zinc finger domains at the C-terminus, as well as a kruppel association box (KRAB) domain (PMID: 10567577). ZNF263 has been reported to be involved in early human embryonic development, cardiac regeneration, and adipogenesis (PMID: 37065635).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Front Oncol; Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.; Authors - Min-Yun Cai
human	WB,ChIP	Pharmacogenomics J; The ZNF263/CPT1B axis regulates fatty acid β-oxidation to affect cisplatin resistance in lung adenocarcinoma; Authors - Renhe Yan