ZNF277 Polyclonal antibody

ZNF277 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25094-1-AP

Synonyms

NRIF4, zinc finger protein 277, ZNF277, ZNF277P

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Validation Data Gallery

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  WB analysis of PC-3 using 25094-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25094-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 25094-1-AP

  Immunofluorescent analysis of HepG2 cells using 25094-1-AP (ZNF277 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: PC-3 cells, Jurkat cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25094-1-AP is ZNF277 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF277 fusion protein Ag18979
• Full Name: zinc finger protein 277
• Calculated Molecular Weight: 450 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC020626
• Gene Symbol: ZNF277
• Gene ID (NCBI): 11179
• RRID: AB_2879893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.