ZNF281 Polyclonal antibody

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• ZNF281 Polyclonal antibody (24088-1-AP)

• 

  WB analysis using 24088-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24088-1-AP (ZNF281 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 24088-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF281 antibody (24088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

ZNF281 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24088-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GC-box-binding zinc finger protein 1, GZP1, Transcription factor ZBP-99, ZBP 99, ZBP99

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Tested Applications

• Positive WB detected in: NCCIT cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24088-1-AP targets ZNF281 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21355

  Product name: Recombinant human ZNF281 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 695-895 aa of BC060820

  Sequence: LSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

• Full Name: zinc finger protein 281
• Calculated Molecular Weight: 895 aa, 97 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC060820
• Gene Symbol: ZNF281
• Gene ID (NCBI): 23528
• RRID: AB_3085722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9Y2X9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF281 (zinc finger protein 281) is a Krüppel family transcription factor containing four C2H2-type zinc finger domains, located in the cell nucleus. It promotes epithelial-mesenchymal transition (EMT) and tumor metastasis by recruiting co-repressors such as CTBP or synergistically with ZEB1 to inhibit the expression of epithelial markers such as E-cadherin. Furthermore, this protein is also involved in maintaining embryonic stem cell pluripotency and regulating neural differentiation.