ZNF3 Polyclonal antibody

ZNF3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16950-1-AP

Synonyms

A8 51, HF.12, KOX25, PP838, Zfp113, zinc finger protein 3, Zinc finger protein HF.12, Zinc finger protein HZF3.1, Zinc finger protein KOX25, ZNF3

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Validation Data Gallery

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  WB analysis of mouse ovary using 16950-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16950-1-AP (ZNF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16950-1-AP (ZNF3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16950-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16950-1-AP (ZNF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 16950-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 16950-1-AP (ZNF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 16950-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16950-1-AP (ZNF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse ovary tissue, mouse testis tissue, A2780 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

16950-1-AP targets ZNF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF3 fusion protein Ag10578
• Full Name: zinc finger protein 3
• Calculated Molecular Weight: 446 aa, 51 kDa
• Observed Molecular Weight: 47-51 kDa
• GenBank Accession Number: BC013603
• Gene Symbol: ZNF3
• Gene ID (NCBI): 7551
• RRID: AB_2218261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF3 is predicted to be involved in negative regulation of transcription by RNA polymerase II, and also involved in cell differentiation and/or proliferation.

Publications

Species	Application	Title
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); ZNF3 regulates proliferation, migration and invasion through MMP1 and TWIST in colorectal cancer; Authors - Le Du