Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25147-1-AP targets ZNF326 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18433 Product name: Recombinant human ZNF326 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-486 aa of BC090866 Sequence: MGDFGSIHRPGIVVDYQNKSTNVTVAAARGIKRKMMQPFNKPSGTFIKKPKLAKPMEKISLSKSPTKTDPKNEEEEKRRIEARREKQRRRREKNSEKYGDGYRMAFTCSFCKFRTFEEKDIELHLESSSHQETLDHIQKQTKFDKVVMEFLHECMVNKFKKTSIRKQQTNNQTEVVKIIEKDVMEGVTVDDHMMKVETVHCSACSVYIPALHSSVQQHLKSPDHIKGKQAYKEQIKRESVLTATSILNNPIVKARYERFVKGENSFEIQDHSQDQQIEGD Predict reactive species |
| Full Name | zinc finger protein 326 |
| Calculated Molecular Weight | 582 aa, 66 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC090866 |
| Gene Symbol | ZNF326 |
| Gene ID (NCBI) | 284695 |
| RRID | AB_2879923 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5BKZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZNF326 antibody 25147-1-AP | Download protocol |
| IHC protocol for ZNF326 antibody 25147-1-AP | Download protocol |
| WB protocol for ZNF326 antibody 25147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZNF326 Polyclonal antibody (Cat# 25147-1-AP) has 3 total citations. These appear in Journal of Translational Medicine, Toxicological Sciences, and Scientific Reports. The research fields span colorectal cancer progression and immune evasion, paraquat-induced neurotoxicity, and TDP-43 aggregation-related proteomics. All citations used WB; one additionally employed IF.
| Species | Application | Title |
|---|---|---|
J Transl Med Cathepsin E drives colorectal cancer progression and immune evasion via ZNF326 ADP-ribosylation and TGF-β/SMAD activation. | ||
Toxicol Sci LncRNA NR_030777 Alleviates Paraquat-induced Neurotoxicity by Regulating Zfp326 and Cpne5. | ||
Sci Rep TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot at a 1:2000 dilution (lot 00055260). One user tested it on RKO cells and confirmed ZNF326 is visible below 75 kDa, noting some weak non-specific bands. The other tested it on U2OS cells and confirmed detection of the band at the correct molecular weight. Overall, users find the antibody reliable for WB with good specificity at the expected size.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aleksandr (Verified Customer) (12-16-2024) | Great! Znf326 is visible (below 75 kDa), as well as other weak non-specific bands
 |
Tsimafei (Verified Customer) (08-07-2024) | Antibody detect the band of the correct size
 |
