ZNF326 Polyclonal antibody

ZNF326 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25147-1-AP

Publications (3)

Reviews (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DBIRD complex subunit ZNF326, ZAN75, Zfp326, Zinc finger protein 326, Zinc finger protein interacting with mRNPs and DBC1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Proteintech Guarantee

Filter: AllWBIHCIF/ICC

WB analysis of Jurkat using 25147-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 25147-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 25147-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 25147-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25147-1-AP (ZNF326 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 25147-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 25147-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25147-1-AP (ZNF326 Antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 25147-1-AP
Immunofluorescent analysis of HeLa cells using 25147-1-AP (ZNF326 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols
Reviews (2)

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected in	mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25147-1-AP targets ZNF326 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18433 Product name: Recombinant human ZNF326 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-486 aa of BC090866 Sequence: MGDFGSIHRPGIVVDYQNKSTNVTVAAARGIKRKMMQPFNKPSGTFIKKPKLAKPMEKISLSKSPTKTDPKNEEEEKRRIEARREKQRRRREKNSEKYGDGYRMAFTCSFCKFRTFEEKDIELHLESSSHQETLDHIQKQTKFDKVVMEFLHECMVNKFKKTSIRKQQTNNQTEVVKIIEKDVMEGVTVDDHMMKVETVHCSACSVYIPALHSSVQQHLKSPDHIKGKQAYKEQIKRESVLTATSILNNPIVKARYERFVKGENSFEIQDHSQDQQIEGD Predict reactive species
Full Name	zinc finger protein 326
Calculated Molecular Weight	582 aa, 66 kDa
Observed Molecular Weight	66-70 kDa
GenBank Accession Number	BC090866
Gene Symbol	ZNF326
Gene ID (NCBI)	284695
RRID	AB_2879923
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5BKZ1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ZNF326 antibody 25147-1-AP	Download protocol
IHC protocol for ZNF326 antibody 25147-1-AP	Download protocol
WB protocol for ZNF326 antibody 25147-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ZNF326 Polyclonal antibody (Cat# 25147-1-AP) has 3 citations spanning three journals: J Transl Med, Toxicol Sci, and Sci Rep. The associated research fields include colorectal cancer progression and immune evasion (via ZNF326 ADP-ribosylation and TGF-β/SMAD signaling), paraquat-induced neurotoxicity (regulating Zfp326 and Cpne5), and TDP-43 aggregation in neurodegeneration. All citations used WB, with one also employing IF, across human and mouse species.

Species	Application	Title
human	WB	J Transl Med Cathepsin E drives colorectal cancer progression and immune evasion via ZNF326 ADP-ribosylation and TGF-β/SMAD activation. Authors - Huan Zhang View Article
mouse	WB	Toxicol Sci LncRNA NR_030777 Alleviates Paraquat-induced Neurotoxicity by Regulating Zfp326 and Cpne5. Authors - Hongyu Yang View Article
human	WB,IF	Sci Rep TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. Authors - S Prpar Mihevc View Article

Reviews

5.0 out of 5

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2 verified customer reviews

What customers say

Both reviewers rated the antibody 5 stars for Western Blot. One user tested it on RKO cells and confirmed a clear ZNF326 signal below 75 kDa, noting a few weak non-specific bands. The other tested it on U2OS cells and confirmed detection of a band at the expected molecular weight. Both used a 1:2000 dilution with the same lot. Overall, the antibody performs reliably at the correct size with minimal background.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Aleksandr (Verified Customer) (12-16-2024)	Great! Znf326 is visible (below 75 kDa), as well as other weak non-specific bands Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: RKO ZNF326 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:25147-1-AP)
Tsimafei (Verified Customer) (08-07-2024)	Antibody detect the band of the correct size Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: U2OS ZNF326 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:25147-1-AP)