ZNF34 Polyclonal antibody

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  WB analysis of HeLa using 20477-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20477-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20477-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20477-1-AP (ZNF34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 20477-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20477-1-AP (ZNF34 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20477-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20477-1-AP (ZNF34 antibody) at dilution of 1:50 (under 40x lens).

ZNF34 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20477-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KOX32, zinc finger protein 34, Zinc finger protein KOX32, ZNF34

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20477-1-AP targets ZNF34 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14346

  Product name: Recombinant human ZNF34 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC004480

  Sequence: MAALFLSAPPQAEVTFEDVAVYLSREEWGRLGPAQRGLYRDVMLETYGNLVSLGVGPAGPKPGVISQLERGDEPWVLDVQGTSGKEHLRVNSPALGTRTEYKELTSQETFGEEDPQGSEPVEACDHISKSEGSLEKLVEQRGPRAVTLTNGESSRESGGNLRLLSRPVPDQRPHKCDICEQSFEQRSYLNNHKRVHRSKKTNTVRNSGEIFSANLVVKEDQKIPTGKKLHYCSYCGKTFRYSANLVKHQRLHTEEKPYKCDECGKAFSQSCEFINHRRMHSGEIPYRCDECGKTFTRRPNLMKHQRIHTGEKPYKCGECGKHFSAYSSLIYHQRIHTGEKPYKCNDCGKA

• Full Name: zinc finger protein 34
• Calculated Molecular Weight: 539aa,62 kDa; 560aa,64 kDa
• Observed Molecular Weight: 62-68 kDa
• GenBank Accession Number: BC004480
• Gene Symbol: ZNF34
• Gene ID (NCBI): 80778
• RRID: AB_10665367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZ26
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF34, also named as KOX32, contains twelve C2H2-type zinc fingers and one KRAB domain. It belongs to the krueppel C2H2-type zinc-finger protein family. This protein may be involved in transcriptional regulation.