ZNF346 Polyclonal antibody

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ZNF346 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20794-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Zinc finger protein 346, Zfp346, JAZ

• 

  WB analysis of human brain using 20794-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20794-1-AP (ZNF346 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 20794-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20794-1-AP (ZNF346 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 20794-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20794-1-AP (ZNF346 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 20794-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20794-1-AP (ZNF346 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 20794-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20794-1-AP (ZNF346 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 20794-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF346 antibody (20794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human testis tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20794-1-AP targets ZNF346 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14585

  Product name: Recombinant human ZNF346 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-294 aa of BC007775

  Sequence: MEYPAPATVQAADGGAAGPYSSSELLEGQEPDGVRFDRERARRLWEAVSGAQPVGREEVEHMIQKNQCLFTNTQCKVCCALLISESQKLAHYQSKKHANKVKRYLAIHGMETLKGETKKLDSDQKSSRSKDKNQCCPICNMTFSSPVVAQSHYLGKTHAKNLKLKQQSTKVEALHQNREMIDPDKFCSLCHATFNDPVMAQQHYVGKKHRKQETKLKLMARYGRLADPAVTDFPAGKGYPCKTCKIVLNSIEQYQAHVSGFKHKNQSPKTVASSLGQIPMQRQPIQKDSTTLED

• Full Name: zinc finger protein 346
• Calculated Molecular Weight: 294 aa, 33 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC007775
• Gene Symbol: ZNF346
• Gene ID (NCBI): 23567
• RRID: AB_10695766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL40
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The ZNF346 Polyclonal antibody (Cat# 20794-1-AP) has a total of 1 citation. The cited publication appeared in International Journal of Molecular Sciences (2023), investigating ZNF346 as a prognostic biomarker associated with an inflamed immunosuppressive tumor microenvironment in hepatocellular carcinoma. The research field encompasses cancer biology, specifically liver cancer biomarker discovery and tumor immunology. The antibody was used for Western blot in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; ZNF385A and ZNF346 Serve as Prognostic Biomarkers Associated with an Inflamed Immunosuppressive Tumor Microenvironment in Hepatocellular Carcinoma; Authors - Qihang Peng ; KD Validated