ZNF385A Polyclonal antibody

• Featured Product
• KD/KO Validated

ZNF385A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26288-1-AP

Synonyms

ZNF385, Zinc finger protein 385A, ZFP385

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Validation Data Gallery

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  WB analysis of HeLa using 26288-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26288-1-AP (ZNF385A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brown disease using 26288-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26288-1-AP (ZNF385A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brown disease using 26288-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26288-1-AP (ZNF385A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HaCaT using 26288-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using ZNF385A antibody (26288-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human brown disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

26288-1-AP targets ZNF385A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF385A fusion protein Ag23577
• Full Name: zinc finger protein 385A
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC029752
• Gene Symbol: ZNF385A
• Gene ID (NCBI): 25946
• RRID: AB_2880464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; ZNF385A and ZNF346 Serve as Prognostic Biomarkers Associated with an Inflamed Immunosuppressive Tumor Microenvironment in Hepatocellular Carcinoma; Authors - Qihang Peng ; KD Validated
human,mouse	WB,IHC	J Proteome Res; Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A; Authors - Qihang Peng ; KD Validated