ZNF394 Polyclonal antibody

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• ZNF394 Polyclonal antibody (25785-1-AP)

ZNF394 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25785-1-AP Copied!

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ZKSCAN14

• 

  WB analysis of mouse brain using 25785-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of fetal human brain using 25785-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 25785-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 25785-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human urothelial carcinoma using 25785-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25785-1-AP (ZNF394 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue, HepG2 cells, mouse spleen tissue
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25785-1-AP targets ZNF394 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22655

  Product name: Recombinant human ZNF394 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 140-375 aa of BC051847

  Sequence: RALQRALDGTSSQGMVTFEDTAVSLTWEEWERLDPARRDFCRESAQKDSGSTVPPSLESRVENKELIPMQQILEEAEPQGQLQEAFQGKRPLFSKCGSTHEDRVEKQSGDPLPLKLENSPEAEGLNSISDVNKNGSIEGEDSKNNELQNSARCSNLVLCQHIPKAERPTDSEEHGNKCKQSFHMVTWHVLKPHKSDSGDSFHHSSLFETQRQLHEERPYKCGNCGKSFKQRSDLFR

• Full Name: zinc finger protein 394
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC051847
• Gene Symbol: ZNF394
• Gene ID (NCBI): 84124
• RRID: AB_2880239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53GI3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF394 (zinc finger protein 394) is a C2H2-type zinc finger protein belonging to the Krüppel C2H2-type zinc finger protein family. The protein contains three distinct domains: the N-terminus has two domains, LER and KRAB, and the C-terminus has seven C2H2 zinc finger domains. ZNF394 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZNF394, together with ZIM3, plays a key role in human embryonic development at the 8-cell stage by inhibiting the expression of genes specific to the 4-cell stage, thereby promoting embryonic development.

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

One reviewer rated this product 5 stars. They used it at a 1:1000 dilution for Western Blot on HEK293T cells (lot AG22655), preparing the sample from a 6-well plate pellet lysed with 1 mL of NP40 buffer. The review was posted in April 2022 and reflects a positive experience with the antibody's performance in their workflow.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Simone (Verified Customer) (04-25-2022)

From 6 well plate pellet, lysed with 1ml NP40 buffer

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells