ZNF397 Polyclonal antibody

ZNF397 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20799-1-AP

Synonyms

zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15

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Validation Data Gallery

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  WB analysis of PC-3 using 20799-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20799-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20799-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 20799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse testis tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20799-1-AP targets ZNF397 in WB, IHC, ELISA applications and shows reactivity with human, mouse, Rat samples.

• Tested Reactivity: human, mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF397 fusion protein Ag14712
• Full Name: zinc finger protein 397
• Calculated Molecular Weight: 534 aa, 61 kDa
• Observed Molecular Weight: 60-63 kDa
• GenBank Accession Number: BC006172
• Gene Symbol: ZNF397
• Gene ID (NCBI): 84307
• RRID: AB_2878741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF397 (Zinc Finger Protein 397) is conserved in placental mammals. ZNF397 as a bona fide coactivator of the androgen receptor (AR), essential for the transcriptional program governing AR-driven luminal lineage.