Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse brain tissue, HeLa cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14529-1-AP targets ZNF410 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6028 Product name: Recombinant human ZNF410 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC050683 Sequence: MLSDELESKPELLVQFVQNTSIPLGQGLVESEAKDITCLSLLPVTEASECSRLMLPDDTTNHSNSSKEVPSSAVLRSLRVNVGPDGEETRAQTVQKSPEFLSTSESSSLLQDLQPSDSTSFILLNLTRAGLGSSAEHLVFVQDEAEDSGNDFLSSESTDSSIPWFLRVQELAHDSLIAATRAQLAKNAKTSSNGENVHLGSGDGQSKDSGPLPQVEKKLKCTVEGCDRTFVWPAHFKYHLKTHRNDRSFICPAEGCGKSFYVLQRLKVHMRTHNGEKPFMCHESGCGKQFTTAGNLKNHRRIHTGEKPFLCEAQGCGRSFAEYSSLRKHLVVHSGEKPHQCQVCGKTFSQS Predict reactive species |
| Full Name | zinc finger protein 410 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 52-67 kDa |
| GenBank Accession Number | BC050683 |
| Gene Symbol | ZNF410 |
| Gene ID (NCBI) | 57862 |
| RRID | AB_2257520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF410 (Zinc Finger Protein 410) is a transcription factor involved in the regulation of gene expression, particularly in the context of fetal hemoglobin (HbF) repression and chromatin remodeling. This protein is highly conserved among mammals and plays a crucial role in erythroid cells. The study found that ZNF410 uniquely silences HbF by activating its sole target gene, CHD4, a known HbF repressor in erythroid cells. (PMID: 40158221)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF410 antibody 14529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZNF410 Polyclonal antibody (Cat# 14529-1-AP) has 9 total citations. These appear in journals including Nature Genetics, Molecular Cell, Molecular Therapy, EMBO Journal, Free Radical Biology and Medicine, Cell Reports, Biomedicines, and bioRxiv. The associated research fields span fetal hemoglobin/globin regulation (BCL11A, CHD4/NuRD), β-hemoglobinopathy therapy, transposable element expression in early embryos, brown adipose tissue formation, intestinal ischemia-reperfusion, chromatin accessibility/enhancer activity, and lymphangiogenesis. Applications were primarily Western blot, with one immunofluorescence and one ChIP study.
| Species | Application | Title |
|---|---|---|
Mol Cell ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression. | ||
Mol Ther Development of a double shmiR lentivirus effectively targeting both BCL11A and ZNF410 for enhanced induction of fetal hemoglobin to treat β-hemoglobinopathies. | ||
EMBO J PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase. | ||
Free Radic Biol Med Critical role of caveolin-1 in intestinal ischemia reperfusion by inhibiting protein kinase C βII |
