Tested Applications
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32701-1-AP targets ZNF449 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36778 Product name: Recombinant human ZNF449 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-430 aa of BC060768 Sequence: ENREEPWVKELQDSKEMKQLLDSKIGFEIGIENEEDTSKQKKMETMYPFIVTLEGNALQGPILQKDYVQLENQWETPPEDLQTDLAKLVDQQNPTLGETPENSNLEEPLNPKPHKKKSPGEKPHRCPQCGKCFARKSQLTGHQRIHSGEEPHKCPECGKRFLRSSDLYRHQRLHTGERPYECTVCKKRFTRRSHLIGHQRTHSEEETYKCLECGKSFCHGSSLKRHLKTHT Predict reactive species |
| Full Name | zinc finger protein 449 |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC060768 |
| Gene Symbol | ZNF449 |
| Gene ID (NCBI) | 203523 |
| RRID | AB_3742715 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6P9G9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF449 (Zinc Finger Protein 449) is a member of the zinc-finger family of transcription factors. It is encoded by a gene located on the X chromosome (Xq26.3) and contains an N-terminal SCAN domain and seven C2H2-type zinc finger motifs at the C-terminus.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZNF449 antibody 32701-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
