ZNF488 Polyclonal antibody

ZNF488 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21014-1-AP

Synonyms

zinc finger protein 488, ZNF488

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Validation Data Gallery

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  WB analysis of mouse thymus using 21014-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21014-1-AP (ZNF488 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 21014-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21014-1-AP (ZNF488 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21014-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21014-1-AP (ZNF488 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 21014-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21014-1-AP (ZNF488 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse thymus tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 21014-1-AP is ZNF488 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF488 fusion protein Ag15239
• Full Name: zinc finger protein 488
• Calculated Molecular Weight: 340 aa, 37 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC026895
• Gene Symbol: ZNF488
• Gene ID (NCBI): 118738
• RRID: AB_10693631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,WB	Biol Direct; Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism; Authors - Qifeng Xiao ; KD Validated