Tested Applications
| Positive WB detected in | mouse thymus tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21014-1-AP targets ZNF488 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15239 Product name: Recombinant human ZNF488 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-233 aa of BC026895 Sequence: MKDRQVDAQAQEREHDDPTGQPGAPQLTQNIPRGPAGSKVFSVWPSGARSEQRSAFSKPTKRPAERPELTSVFPAGESADALGELSGLLNTTDLACWGRLSTPKLLVGDLWNLQALPQNAPLCSTFLGAPTLWLEHTQAQVPPPSSSSTTSWALLPPTLTSLGLSTQNWCAKCNLSFRLTSDLVFHMRSHHKKEHAGPDPHSQKRREEALACPVCQEHFRERHHLSRHMTSHS Predict reactive species |
| Full Name | zinc finger protein 488 |
| Calculated Molecular Weight | 340 aa, 37 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC026895 |
| Gene Symbol | ZNF488 |
| Gene ID (NCBI) | 118738 |
| RRID | AB_10693631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF488 antibody 21014-1-AP | Download protocol |
| WB protocol for ZNF488 antibody 21014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZNF488 Polyclonal antibody (Cat# 21014-1-AP) has 1 citation. It was published in Biol Direct (2023), a study investigating how ZNF488 overexpression promotes pancreatic cancer cell proliferation and tumorigenesis by inhibiting ferroptosis through SCD1-mediated unsaturated fatty acid metabolism regulation. The antibody was used for IHC and WB in human samples. The research falls within the fields of pancreatic cancer biology, ferroptosis, and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Biol Direct Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism
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