Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31300-1-AP targets ZNF638 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35296 Product name: Recombinant human ZNF638 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1777-1978 aa of NM_014497.4 Sequence: VDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR Predict reactive species |
| Full Name | zinc finger protein 638 |
| Calculated Molecular Weight | 221kDa |
| Observed Molecular Weight | 270-300 kDa |
| GenBank Accession Number | NM_014497.4 |
| Gene Symbol | ZNF638 |
| Gene ID (NCBI) | 27332 |
| RRID | AB_3669937 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q14966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404). The ZNF638 protein is modified and exhibits a molecular weight of ~300 kDa (PMID: 33040080)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZNF638 antibody 31300-1-AP | Download protocol |
| WB protocol for ZNF638 antibody 31300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Moritz (Verified Customer) (09-26-2025) | Several band are visible in the area between 150-250 kDa
 |
FH Aleksandr (Verified Customer) (07-29-2025) | Antibody recognise a band of the expected size with some other prominent, likely non-specific ones
 |
FH Tsimafei (Verified Customer) (08-07-2024) | Great antibody
 |
FH Julia (Verified Customer) (08-07-2024) | Band below 250 kDa marker is likely the Znf639 (~221kDa), there is also strong signal between 100-50kDa. I used 30ug of mESC protein extract.
 |
