ZNF638 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84359-3-RR (same clone as 84359-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84359-3-RR (ZNF638 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84359-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84359-3-RR (same clone as 84359-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF638 antibody (84359-3-RR, Clone: 241636C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84359-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84359-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84359-3-RR against Human ZNF638 were performed. The affinity constant is below 1 pM.

ZNF638 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84359-3-PBS

Clone No.241636C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

zinc finger protein 638, ZFML, Nuclear protein 220, NP220, CTCL associated antigen se33 1

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Product Information

84359-3-PBS targets ZNF638 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag35296

  Product name: Recombinant human ZNF638 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1777-1978 aa of NM_014497.4

  Sequence: VDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR

• Full Name: zinc finger protein 638
• Calculated Molecular Weight: 221kDa
• Observed Molecular Weight: 270-300 kDa
• GenBank Accession Number: NM_014497.4
• Gene Symbol: ZNF638
• Gene ID (NCBI): 27332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14966
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404).