ZNF639 Polyclonal antibody

ZNF639 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25817-1-AP

Synonyms

6230400O18Rik, ANC 2H01, ANC_2H01, ZASC1, zinc finger protein 639, Zinc finger protein ANC_2H01, Zinc finger protein ZASC1, ZNF639

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Validation Data Gallery

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  WB analysis of HeLa using 25817-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25817-1-AP (ZNF639 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25817-1-AP

  IP result of anti-ZNF639 (IP:25817-1-AP, 4ug; Detection:25817-1-AP 1:300) with HeLa cells lysate 3960ug.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25817-1-AP is ZNF639 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF639 fusion protein Ag19854
• Full Name: zinc finger protein 639
• Calculated Molecular Weight: 485 aa, 56 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC026181
• Gene Symbol: ZNF639
• Gene ID (NCBI): 51193
• RRID: AB_2880251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF639, also named as ZASC1, is a 485 amino acid protein, which locates in the nucleus and contains 8 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF639 binds DNA and may function as a transcriptional repressor.