ZNF654 Polyclonal antibody

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• ZNF654 Polyclonal antibody (24596-1-AP)

ZNF654 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24596-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Melanoma associated antigen, zinc finger protein 654, ZNF654

• 

  WB analysis of mouse ovary using 24596-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 24596-1-AP (ZNF654 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 24596-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24596-1-AP (ZNF654 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse ovary using 24596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24596-1-AP (ZNF654 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse ovary using 24596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24596-1-AP (ZNF654 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 24596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF654 antibody (24596-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse ovary tissue, MCF-7 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24596-1-AP targets ZNF654 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20186

  Product name: Recombinant human ZNF654 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 326-581 aa of BC153004

  Sequence: TPESSAQPSETILWDVQTDSNPNQEKDSSSNEKQTISLPVSTSKSRKESTEPKTCIESMEKKTDSLVQNGNERSDDTVSNISLIDQKMPDIEPNSENNCSSSDIVNGHSEIEQTPLVSSDPALKIDTNRIRTENGSILPSVVPQEHNTLPVSQAPSKPNLTSEHTSYGLILTKPYVRPLPPSYLDERYLSMPKRRKFLTDRVDACSDQDNVYKKSVKRLRCGKCLTTYCNAEALEAHLAQKKCQTLFGFDSDDESA

• Full Name: zinc finger protein 654
• Calculated Molecular Weight: 581 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC153004
• Gene Symbol: ZNF654
• Gene ID (NCBI): 55279
• RRID: AB_2879630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZM8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The ZNF654 Polyclonal antibody (Cat# 24596-1-AP) has 1 total citation. It appears in Cell Reports (IF 7.7), published in 2026. The cited study investigates how RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency, placing this citation in the research fields of stem cell biology, epigenetics, and transcriptional regulation. The antibody was used in Western Blot (WB) with mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Rep; RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.; Authors - Takamasa Ito