ZNF746 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZNF746 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 24543-1-AP

Synonyms

PARIS, zinc finger protein 746, ZNF746

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Validation Data Gallery

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  WB analysis using 24543-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24543-1-AP (ZNF746 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 24543-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 24543-1-AP (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

24543-1-AP targets ZNF746 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF746 fusion protein Ag21566
• Full Name: zinc finger protein 746
• Calculated Molecular Weight: 644 aa, 69 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC068505
• Gene Symbol: ZNF746
• Gene ID (NCBI): 155061
• RRID: AB_2879598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN (PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).

Publications

Species	Application	Title
mouse	WB	Neuron; Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease.; Authors - Nikhil Panicker ; KO Validated
human,mouse	WB	Sci Adv; Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease.; Authors - Sheila K Pirooznia
mouse	WB	Brain; Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease.; Authors - Saurav Brahmachari
human,mouse	WB,IF	Brain; Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.; Authors - Hyojung Kim
mouse	WB	Front Aging Neurosci; Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice; Authors - Hyojung Kim
human,mouse	WB,IHC	Biochem Biophys Res Commun; ZNF746/PARIS promotes the occurrence of hepatocellular carcinoma.; Authors - Hanna Kim ; KO Validated