ZNF787 Polyclonal antibody

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  WB analysis using 31934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31934-1-AP (ZNF787 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNF787 antibody (31934-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

ZNF787 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31934-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Zinc finger protein 787, TTF-I-interacting peptide 20, Transcription Termination Factor I Interacting Peptide 20, TIP20

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, U-251 cells, RT-4 cells, NIH/3T3 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31934-1-AP targets ZNF787 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF787 fusion protein Ag36052
• Full Name: zinc finger protein 787
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC077728
• Gene Symbol: ZNF787
• Gene ID (NCBI): 126208
• RRID: AB_3670151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6DD87
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 787 (ZNF787, also known as TIP20) is activated in the nucleus and may be involved in transcriptional regulation. It probably is one repressor of neuronal features and can interact with histone deacetylase 1 (HDAC1) in regulating the permeability of the blood-brain barrier (BBB) as well as the pathogenesis of Alzheimer's disease (PMID: 33057419; 38329647).