ZNF8 Polyclonal antibody, PBS Only

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• ZNF8 Polyclonal antibody, PBS Only (17448-1-PBS)

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  WB analysis using 17448-1-AP (same clone as 17448-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17448-1-AP (ZNF8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 17448-1-AP (same clone as 17448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17448-1-AP (ZNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 17448-1-AP (same clone as 17448-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 17448-1-AP (same clone as 17448-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17448-1-PBS in a different storage buffer formulation.

ZNF8 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 17448-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Zfp128, Zinc finger protein 8

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Product Information

17448-1-PBS targets ZNF8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11571

  Product name: Recombinant human ZNF8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 231-575 aa of BC039323

  Sequence: NSDCHRDSSQAIPITELTKSQVQDKPYKCTDCGKSFNHNAHLTVHKRIHTGERPYMCKECGKAFSQNSSLVQHERIHTGDKPYKCAECGKSFCHSTHLTVHRRIHTGEKPYECQDCGRAFNQNSSLGRHKRTHTGEKPYTCSVCGKSFSRTTCLFLHLRTHTEERPYECNHCGKGFRHSSSLAQHQRKHAGEKPFECRQRLIFEQTPALTKHEWTEALGCDPPLSQDERTHRSDRPFKCNQCGKCFIQSSHLIRHQITHTREEQPHGRSRRREQSSSRNSHLVQHQHPNSRKSSAGGAKAGQPESRALALFDIQKIMQEKNPVHVIGVEEPSVGASMLFDIREST

• Full Name: zinc finger protein 8
• Calculated Molecular Weight: 575 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC039323
• Gene Symbol: ZNF8
• Gene ID (NCBI): 7554
• RRID: AB_3669275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17098
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZNF8 is a transcriptional repressor. It may modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.