Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, PC-12 cells, MDA-MB-231 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16595-1-AP targets ZNHIT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9741 Product name: Recombinant human ZNHIT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC017333 Sequence: MVEKKTSVRSQDPGQRRVLDRAARQRRINRQLEALENDNFQDDPHAGLPQLGKRLPQFDDDADTGKKKKKTRGDHFKLRFRKNFQALLEEQNLSVAEGPNYLTACAGPPSRPQRPFCAVCGFPSPYTCVSCGARYCTVRCLGTHQETRCLKWTV Predict reactive species |
| Full Name | zinc finger, HIT type 1 |
| Calculated Molecular Weight | 154 aa, 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC017333 |
| Gene Symbol | ZNHIT1 |
| Gene ID (NCBI) | 10467 |
| RRID | AB_2220382 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43257 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNHIT1(Zinc finger HIT domain containing protein 1), aslo named CGBP1 (Cyclin G1 binding protein 1), is a subunit of the SNF-2 related CBP activator protein (SRCAP) complex, which can remodel chromatin by incorporating the histone variant H2A.Z into nucleosomes(PMID: 15647280). ZNHIT1 also can bind to myogenin promoter and mediate H2A.Z incorporation for its expression(PMID: 20473270). ZNHIT1 overexpression induces apoptosis and represses proliferation and invasion of breast cancer cells(PMID: 30928405).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZNHIT1 antibody 16595-1-AP | Download protocol |
| IHC protocol for ZNHIT1 antibody 16595-1-AP | Download protocol |
| WB protocol for ZNHIT1 antibody 16595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ZNHIT1 Polyclonal antibody (Cat# 16595-1-AP) has 5 total citations. These appear in Nature, Cell Discov, Molecular Pain, bioRxiv, and eLife. The research fields span chromatin remodeling and histone variant H2A.Z exchange by the SRCAP complex, structural biology of histone chaperones, NOD-like receptor signaling in migraine-related hyperalgesia, and DNA methylation's impact on H2A.Z deposition and nucleosome stability.
| Species | Application | Title |
|---|---|---|
Mol Pain Znhit1 alleviates nitroglycerin-induced hyperalgesia in migraine mice via the inhibition of NOD-like receptor signaling pathway. | ||
