ZNHIT2 Polyclonal antibody

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  WB analysis using 16885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16885-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16885-1-AP (ZNHIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZNHIT2 Polyclonal Antibody for WB, ELISA

Cat No. 16885-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

C11orf5, FON, Protein FON, zinc finger, HIT type 2, ZNHIT2

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse brain tissue, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16885-1-AP targets ZNHIT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10351

  Product name: Recombinant human ZNHIT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-403 aa of BC052240

  Sequence: MEPAGPCGFCPAGEVQPARYTCPRCNAPYCSLRCYRTHGTCAENFYRDQVLGELRGCSAPPSRLASALRRLRQQRETEDEPGEAGLSSGPAPGGLSGLWERLAPGEKAAFERLLSRGEAGRLLPPWRPWWWNRGAGPQLLEELDNAPGSDAAELELAPARTPPDSVKDASAAEPAAAERVLGDVPGACTPVVPTRIPAIVSLSRGPVSPLVRFQLPNVLFAYAHTLALYHGGDDALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGEHPPGPLGTRGAMHEVARILLGEGPTNQKGYTLAALGDLAQTLGRARKQAVAREERDHLYRARKKCQFLLAWTNENEAALTPLALDCARAHQAHAVVAEEVAALTGELERLWGGPVPPAPRTLIEELPS

• Full Name: zinc finger, HIT type 2
• Calculated Molecular Weight: 403 aa, 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC052240
• Gene Symbol: ZNHIT2
• Gene ID (NCBI): 741
• RRID: AB_2220389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHR6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNHIT2 belongs to a family of HIT-finger proteins characterized by the presence of a HIT domain, a roughly 50-residue zinc-binding domain containing conserved cysteine (Cys) and histidine (His) residues. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP. ZNHIT2 is highly expressed in testis.