ZNHIT2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84418-7-RR (same clone as 84418-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84418-7-RR (ZNHIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84418-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84418-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84418-7-RR against Human ZNHIT2 were performed. The affinity constant is below 1 pM.

ZNHIT2 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84418-7-PBS

Clone No.241868B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ZNHIT 2, Zinc finger HIT domain-containing protein 2, FON, C11orf5, 241868B10

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Product Information

84418-7-PBS targets ZNHIT2 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10351

  Product name: Recombinant human ZNHIT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-403 aa of BC052240

  Sequence: MEPAGPCGFCPAGEVQPARYTCPRCNAPYCSLRCYRTHGTCAENFYRDQVLGELRGCSAPPSRLASALRRLRQQRETEDEPGEAGLSSGPAPGGLSGLWERLAPGEKAAFERLLSRGEAGRLLPPWRPWWWNRGAGPQLLEELDNAPGSDAAELELAPARTPPDSVKDASAAEPAAAERVLGDVPGACTPVVPTRIPAIVSLSRGPVSPLVRFQLPNVLFAYAHTLALYHGGDDALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGEHPPGPLGTRGAMHEVARILLGEGPTNQKGYTLAALGDLAQTLGRARKQAVAREERDHLYRARKKCQFLLAWTNENEAALTPLALDCARAHQAHAVVAEEVAALTGELERLWGGPVPPAPRTLIEELPS

• Full Name: zinc finger, HIT type 2
• Calculated Molecular Weight: 403 aa, 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC052240
• Gene Symbol: ZNHIT2
• Gene ID (NCBI): 741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UHR6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZNHIT2 belongs to a family of HIT-finger proteins characterized by the presence of a HIT domain, a roughly 50-residue zinc-binding domain containing conserved cysteine (Cys) and histidine (His) residues. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP. ZNHIT2 is highly expressed in testis.