ZNRF2-Specific Polyclonal antibody

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• ZNRF2-Specific Polyclonal antibody (20200-1-AP)

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• KD/KO Validated

ZNRF2-Specific Polyclonal Antibody for WB, ELISA

Cat No. 20200-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Protein Ells2, RING finger protein 202, RNF202, zinc and ring finger 2, Zinc/RING finger protein 2, ZNRF2, ZNRF2-Specific

• 

  WB analysis using 20200-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 20200-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 20200-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue, mouse testis tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20200-1-AP targets ZNRF2-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: zinc and ring finger 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: NM_147128
• Gene Symbol: ZNRF2
• Gene ID (NCBI): 223082
• RRID: AB_2878657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NHG8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNRF2 also named as RNF202, may play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The antibody is specific to ZNRF2.

Publications

What published studies show

The ZNRF2 antibody (Cat# 20200-1-AP) has 4 total citations published in Molecular Neurobiology, Experimental Neurology, Journal of Cellular and Molecular Medicine, and Human Cell. The research fields span neurodegenerative disease (Parkinson's disease and mTOR-mediated neuroinflammation), stroke (cerebral ischemia/reperfusion injury), and oncology (pancancer analysis of ZNRF2 as an oncogene, and its role in breast cancer). All citations used the antibody for Western blot applications across mouse, rat, and human species.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Neurobiol; Deletion of ZNRF2 Exacerbates MPTP-Induced Parkinson's Disease by Activating mTOR-Mediated Neuroinflammatory Pathways; Authors - Fanshi Zhang
rat	WB	Exp Neurol; ZNRF2 attenuates focal cerebral ischemia/reperfusion injury in rats by inhibiting mTORC1-mediated autophagy.; Authors - Chao Gu
human	WB	J Cell Mol Med; A pancancer analysis of the oncogenic role of ZNRF2 in human tumours; Authors - Fujie Shi ; KD Validated
human	WB	Hum Cell; ZNRF2 as an oncogene is transcriptionally regulated by CREB1 in breast cancer models; Authors - Jin-Tao Liu