ZNRF2-Specific Polyclonal antibody

ZNRF2-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20200-1-AP

Synonyms

Protein Ells2, RING finger protein 202, RNF202, zinc and ring finger 2, Zinc/RING finger protein 2, ZNRF2, ZNRF2-Specific

---

Validation Data Gallery

• 

  WB analysis using 20200-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20200-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20200-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue, mouse testis tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

20200-1-AP targets ZNRF2-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: zinc and ring finger 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: NM_147128
• Gene Symbol: ZNRF2
• Gene ID (NCBI): 223082
• RRID: AB_2878657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNRF2 also named as RNF202, may play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The antibody is specific to ZNRF2.

Publications

Species	Application	Title
rat	WB	Exp Neurol; ZNRF2 attenuates focal cerebral ischemia/reperfusion injury in rats by inhibiting mTORC1-mediated autophagy.; Authors - Chao Gu
human	WB	Hum Cell; ZNRF2 as an oncogene is transcriptionally regulated by CREB1 in breast cancer models; Authors - Jin-Tao Liu
human	WB	J Cell Mol Med; A pancancer analysis of the oncogenic role of ZNRF2 in human tumours; Authors - Fujie Shi ; KD Validated