ZO-3 Polyclonal antibody, PBS Only

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• ZO-3 Polyclonal antibody, PBS Only (22116-1-PBS)

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  WB analysis of MCF-7 using 22116-1-AP (same clone as 22116-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

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  WB analysis using 22116-1-AP (same clone as 22116-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 22116-1-AP (same clone as 22116-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

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  IP experiment of HT-29 using 22116-1-AP (same clone as 22116-1-PBS)

  IP result of anti-ZO-3 (IP:22116-1-AP, 4ug; Detection:22116-1-AP 1:1000) with HT-29 cells lysate 1760 ug. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

ZO-3 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 22116-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

Tight junction protein 3, Tight junction protein ZO-3, TJP3, ZO3, Zona occludens protein 3

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Product Information

22116-1-PBS targets ZO-3 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17495

  Product name: Recombinant human TJP3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 84-330 aa of BC108906

  Sequence: SMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPP

• Full Name: tight junction protein 3 (zona occludens 3)
• Calculated Molecular Weight: 952 aa, 105 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC108906
• Gene Symbol: ZO-3
• Gene ID (NCBI): 27134
• RRID: AB_2878998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95049
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-3 (also known as TJP3)is a key scaffold protein of tight junctions. ZO-3 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs).ZO-3 regulates the stability of Cyclin D1, thereby influencing the proliferation of epithelial cells (PMID: 21411630).