ZO-1 Polyclonal antibody
ZO-1 Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 30487-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HT-29 cells, HepG2 cells |
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
30487-1-AP targets ZO-1 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ZO-1 fusion protein Ag33182 |
Full Name | tight junction protein 1 (zona occludens 1) |
Calculated Molecular Weight | 1748 aa, 195 kDa |
Observed Molecular Weight | 230 kDa |
GenBank Accession Number | BC111712 |
Gene Symbol | TJP1 |
Gene ID (NCBI) | 7082 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for ZO-1 antibody 30487-1-AP | Download protocol |
IF protocol for ZO-1 antibody 30487-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Med 5α-Hydroxycostic acid inhibits choroidal neovascularization in rats through a dual signalling pathway mediated by VEGF and angiopoietin 2 |