ZO-1 Polyclonal antibody

ZO-1 Polyclonal Antibody for WB, ELISA, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 30487-1-AP

Synonyms

TJP1, ZO1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1

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Validation Data Gallery

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  WB analysis using 30487-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30487-1-AP (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 30487-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ZO-1 antibody (30487-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HT-29 cells, HUVEC cells, HepG2 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

30487-1-AP targets ZO-1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZO-1 fusion protein Ag33182
• Full Name: tight junction protein 1 (zona occludens 1)
• Calculated Molecular Weight: 1748 aa, 195 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC111712
• Gene Symbol: ZO-1
• Gene ID (NCBI): 7082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).

Publications

Species	Application	Title
rat	WB	Mol Med; 5α-Hydroxycostic acid inhibits choroidal neovascularization in rats through a dual signalling pathway mediated by VEGF and angiopoietin 2; Authors - Wulong Lei
human	WB	Cells; The Natural Product Secoemestrin C Inhibits Colorectal Cancer Stem Cells via p38-S100A8 Feed-Forward Regulatory Loop; Authors - Huimin Zhou