ZP2 Polyclonal antibody

• 

  WB analysis using 21832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21832-1-AP (ZP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse ovary using 21832-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP2 antibody (21832-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZP2 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 21832-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

ZPA, Zp-2, Zp 2, Zona pellucida sperm-binding protein 2, Processed zona pellucida sperm-binding protein 2

---

Tested Applications

• Positive WB detected in: SKOV-3 cells, mouse brain tissue, mouse ovary tissue, rat brain tissue
• Positive IF-P detected in: mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21832-1-AP targets ZP2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16386

  Product name: Recombinant human ZP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 440-745 aa of BC096304

  Sequence: EIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH

• Full Name: zona pellucida glycoprotein 2 (sperm receptor)
• Calculated Molecular Weight: 745 aa, 82 kDa
• Observed Molecular Weight: 75-82 kDa
• GenBank Accession Number: BC096304
• Gene Symbol: ZP2
• Gene ID (NCBI): 7783
• RRID: AB_3669375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05996
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310)