ZPBP Polyclonal antibody

ZPBP Polyclonal Antibody for IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 15835-1-AP

Synonyms

SP38, zona pellucida binding protein, ZPBP, ZPBP1

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Validation Data Gallery

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  IP experiment of mouse testis using 15835-1-AP

  IP result of anti-ZPBP (IP:15835-1-AP, 3ug; Detection:15835-1-AP 1:300) with mouse testis tissue lysate 2800ug.

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Tested Applications

• Positive IP detected in: mouse testis tissue

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15835-1-AP targets ZPBP in IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZPBP fusion protein Ag8585
• Full Name: zona pellucida binding protein
• Calculated Molecular Weight: 351 aa, 40 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC005223
• Gene Symbol: ZPBP
• Gene ID (NCBI): 11055
• RRID: AB_2878189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZPBP (also known as ZPBP1), named for its binding to the oocyte zona pellucida, is a spermatid and spermatozoon protein that localizes to the acrosome. ZPBP may be implicated in gamete interaction during fertilization. It has been reported that absence of ZPBP1 prevents acrosome compaction and leads to acrosome fragmentation and sterility in male mice. (PMID: 17664285; 19204925)