ZRANB2 Polyclonal antibody

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  WB analysis of NIH/3T3 using 24816-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24816-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

ZRANB2 Polyclonal Antibody for WB, ELISA

Cat No. 24816-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ZIS2, ZIS1, ZIS, Zinc finger, splicing, Zinc finger Ran-binding domain-containing protein 2

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

24816-1-AP targets ZRANB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20487

  Product name: Recombinant human ZRANB2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-241 aa of BC039814

  Sequence: MSTKNFRVSDGDWICPDKKCGNVNFARRTSCNRCGREKTTEAKMMKAGGTEIGKTLAEKSRGLFSANDWQCKTCSNVNWARRSECNMCNTPKYAKLEERTGYGGGFNERENVEYIEREESDGEYDEFGRKKKKYRGKAVGPASILKEVEDKESEGEEEDEDEDLSKYKLDEDEDEDDADLSKYNLDASEEEDSNKKKSNRRSRSKSGSSHSRSSSRSSSPSSSRSRSRSRSRSSSSSQSRS

• Full Name: zinc finger, RAN-binding domain containing 2
• Calculated Molecular Weight: 330 aa, 37 kDa
• Observed Molecular Weight: 37 kDa, 48 kDa
• GenBank Accession Number: BC039814
• Gene Symbol: ZRANB2
• Gene ID (NCBI): 9406
• RRID: AB_2879740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95218
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZRANB2, also named as ZIS and ZNF265, is a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. It may interfere with constitutive 5'-splice site selection. The calculated MW of ZRANB2 is 330aa, 37 kDa or 320aa, 36 kDa. We always got the ~48 kDa in our detection. The immunogen of this antibody is 1-241aa. No other protein can be recognized by this antibody from the sequence BLAST results.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nucleic Acids Res; ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.; Authors - Iris Tanaka
human	WB	Aging (Albany NY); LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2.; Authors - Xin Jiang
human	WB,IHC	J Exp Clin Cancer Res; ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.; Authors - Xiaozhi Li ; KD Validated