Tested Applications
| Positive WB detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23111-1-AP targets ZRANB3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19407 Product name: Recombinant human ZRANB3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 728-1079 aa of BC064616 Sequence: LPVYDTLMFCASRNTDRIHIYTKDGKQMSCNFIPLDIKLDLWEDLPASFQLKQYRSLILRFVREWSSLTAMKQRIIRKSGQLFCSPILALEEITKQQTKQNCTKRYITKEDVAVASMDKVKNVGGHVRLITKESRPRDPFTKKLLEDGACVPFLNPYTVQADLTVKPSTSKGYLQAVDNEGNPLCLRCQQPTCQTKQACKANSWDSRFCSLKCQEEFWIRSNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNEMIRNPGEGHFWQVDHIKPVYGGGGQCSLDNLQTLCTVCHKERTARQAKERSQVRRQSLASKHGSDITRFLVKK Predict reactive species |
| Full Name | zinc finger, RAN-binding domain containing 3 |
| Calculated Molecular Weight | 1079 aa, 123 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC064616 |
| Gene Symbol | ZRANB3 |
| Gene ID (NCBI) | 84083 |
| RRID | AB_2744527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5FWF4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZRANB3, also named Annealing helicase 2 or DNA annealing helicase and endonuclease ZRANB3, is a 1079 amino acid protein, which contains one RanBP2-type zinc finger, one helicase C-terminal domain, one HNH domain and one helicase ATP-binding domain. ZRANB3 localizes in the nucleus and belongs to the SNF2/RAD54 helicase family. ZRANB3 as a DNA annealing helicase and endonuclease is required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZRANB3 antibody 23111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ZRANB3 Polyclonal antibody (Cat# 23111-1-AP) has accumulated 22 citations published in journals including Nature, Nature Communications, Molecular Cell, Nucleic Acids Research, Cell Death & Disease, Cell Reports, Journal of Cell Biology, iScience, and bioRxiv. Associated research fields include DNA replication fork reversal, genome stability maintenance, DNA damage response, replication restart mechanisms, R-loop biology, and transcription-replication conflict resolution.
| Species | Application | Title |
|---|---|---|
Nat Commun Rad52 prevents excessive replication fork reversal and protects from nascent strand degradation.
| ||
Nat Commun BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage. | ||
Nat Commun Multifaceted roles of PDS5B in RAD51-dependent homology-directed DNA repair and replication fork protection. | ||
Nat Commun Distinct mechanisms of replication stress induced by oncogenic RAS and cyclin E1 converge on R-loop-dependent fork reversal.
| ||
Nat Commun FET proteins and PARylation-dependent condensates promote replication fork reversal and genome stability. |
