ZSCAN18 Polyclonal antibody

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• ZSCAN18 Polyclonal antibody (30704-1-AP)

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  IHC staining of human stomach using 30704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30704-1-AP (ZSCAN18 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 30704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30704-1-AP (ZSCAN18 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 30704-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ZSCAN18 antibody (30704-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ZSCAN18 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 30704-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

ZSCAN 18

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Tested Applications

• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30704-1-AP targets ZSCAN18 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33264

  Product name: Recombinant human ZSCAN18 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 256-510 aa of BC022846

  Sequence: DAASRLDTEELRLVERDPQGSSLPEGGRRQESAGCACEEAAPAGVLPELPTEAPPGDALADPPSGTTEEEEEQPGKAPDPQDPQDAESDSATGSQRQSVIQQPAPDRGTAKLGTKRPHPEDGDEQSLEGVSSSGDSAGLEAGQGPGADEPGLSRGKPYACGECGEAFAWLSHLMEHHSSHGGRKRYACQGCWKTFHFSLALAEHQKTHEKEKSYALGGARGPQPSTREAQAGARAGGPPESVEGEAPPAPPEAQR

• Full Name: zinc finger and SCAN domain containing 18
• Calculated Molecular Weight: 510 aa, 55 kDa
• GenBank Accession Number: BC022846
• Gene Symbol: ZSCAN18
• Gene ID (NCBI): 65982
• RRID: AB_3742368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TBC5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger and scan domain containing 18 (ZSCAN18) is a ZSCAN TF (a subfamily of ZNF TFs). ZSCAN18 is also known as ZNF447 and is located in chromosome 19q13.43. Recently, hypermethylation in the promoter of ZSCAN18 has been identified in various types of cancers, such as esophageal cancer , renal cell carcinoma, cholangiocarcinoma , colorectal cancer , GC , hepatocellular carcinoma , and pancreatic cancer .(PMID: 36650573, PMID: 40555855)