ZSCAN20 Polyclonal antibody

ZSCAN20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20855-1-AP

Synonyms

KOX29, ZFP 31, Zinc finger protein 31, Zinc finger protein 360, Zinc finger protein KOX29, ZNF31, ZNF360, ZSCAN20

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Validation Data Gallery

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  WB analysis of HepG2 using 20855-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20855-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20855-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 20855-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

• 

  IHC staining of human small intestine using 20855-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:200.

• 

  IHC staining of human small intestine using 20855-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:200.

• 

  IHC staining of human small intestine using 20855-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20855-1-AP is ZSCAN20 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZSCAN20 fusion protein Ag14798
• Full Name: zinc finger and SCAN domain containing 20
• Calculated Molecular Weight: 1043 aa, 118 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC008827
• Gene Symbol: ZSCAN20
• Gene ID (NCBI): 7579
• RRID: AB_2878753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.