ZSCAN29 Polyclonal antibody

ZSCAN29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21630-1-AP

Synonyms

Zfp690, Zinc finger protein 690, ZNF690, ZSCAN29

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Validation Data Gallery

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  WB analysis using 21630-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21630-1-AP (ZSCAN29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21630-1-AP (ZSCAN29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21630-1-AP (ZSCAN29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21630-1-AP targets ZSCAN29 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZSCAN29 fusion protein Ag16239
• Full Name: zinc finger and SCAN domain containing 29
• Calculated Molecular Weight: 852 aa, 97 kDa
• Observed Molecular Weight: 96-97 kDa
• GenBank Accession Number: BC109271
• Gene Symbol: ZSCAN29
• Gene ID (NCBI): 146050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZSCAN29 (zinc finger and SCAN domain containing 29), also named as ZNF690 or Zfp690. It is predicted to be located in nucleus and ubiquitous expression in testis and lymph node. The calculated molecular weight of ZSCAN29 is 96 kDa. The genes ZSCAN29 (associated with five disorders: SCZ, BIP, MD, ASD and ADHD) and ZSCAN31 (associated with three disorders: SCZ, BIP and MD) encode zing finger transcription factor proteins that belong to the krueppel C2H2-type zinc-finger protein family. Zinc finger proteins are essential in multiple regulatory mechanisms and some have been associated with psychiatric disorders as reviewed by Squassina et al., 2019 (PMID: 34637873). It also enables sequence-specific double-stranded DNA binding activity and be predicted to be involved in regulation of transcription by RNA polymerase II.