a-Actinin Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA sample test of MP02360-2

  The mean Alpha Actinin concentration was determined to be 20.47 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 12.30 ng/mL in C6 cell extract based on a 1.20 mg/mL extract load and 28.22 ng/mL in HepG2 cell extract based on a 1.30 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02360-2

  Sandwich ELISA standard curve of MP02360-2, Human Alpha Actinin Recombinant Matched Antibody Pair - PBS only. 86340-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1859. 86340-3-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

a-Actinin Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 86340-4-PBS

Clone No.250833F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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Product Information

86340-4-PBS targets a-Actinin as part of a matched antibody pair:

MP02360-2: 86340-4-PBS capture and 86340-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1859

  Product name: Recombinant human a-Actinin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 543-892 aa of BC015766

  Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

• Full Name: actinin, alpha 1
• Calculated Molecular Weight: 103 kDa
• GenBank Accession Number: BC015766
• Gene Symbol: Alpha Actinin
• Gene ID (NCBI): 87
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12814
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.