ChromoTek Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH, Alexa Fluor® 647 [CTK0107]

Nano-Secondary® alpaca anti-mouse IgG3, Fab-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of a Nanobody that binds to mouse IgG3 with high affinity & specificity.

Reactivity

Mouse

Applications

IF, WB

Conjugate

Alexa Fluor® 647

Cat no : sms3AF647-1

Synonyms

mouse IgG3; mouse immunoglobulin Gamma 3

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Validation Data Gallery

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  Anti-mouse IgG3 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG3 antibody (grey) with 2 copies of a monoclonal mouse Fab- specific Nanobody (green) bound. In total, 6 fluorophores (red stars) label the mouse IgG3 primary antibody.

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  HeLa cells were immunostained with mouse IgG3 anti-Lamin A/C antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor? 647 (magenta). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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  One-step staining (left) vs. sequential staining (right) of HeLa cells with anti-MOT (mitochondria) mouse IgG3 monoclonal primary antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor? 647 (magenta). Cell nuclei are stained with DAPI (blue). Scale bar, 20 μm.

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  Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one conventional secondary antibody. Green: mouse IgG3 anti-Lamin + alpaca anti-mouse IgG3 VHH Alexa Fluor? 488. Red: mouse IgG1 anti-Tubulin + alpaca anti-mouse gG1 VHH Alexa Fluor? 568. Magenta: rabbit anti-Ki67 + conventional polyclonal secondary anti-rabbit-AF647. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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  Higher resolution with anti-mouse IgG3 Nano-Secondary compared to conventional secondary antibodies. Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary antibody.

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Product Information

Nano-Secondary® alpaca anti-mouse IgG3, Fab-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of a Nanobody that binds to mouse IgG3 with high affinity & specificity.

• Applications: IF, WB
• Recommended Dilution: Immunofluorescence 1:1,000; Western blot 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000.; Note: Image acquisition time may have to be optimized.
• Clonality: Monoclonal
• Affinity: CTK0107: KD = 5.7 nM
• Specificity/Target: Mouse IgG3; this Nanobody recognizes the Fab fragment of mouse IgG3
• Reactivity: Mouse
• Conjugate: Alexa Fluor® 647
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.