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ChromoTek Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH, Alexa Fluor® 647 [CTK0107]

Nano-Secondary® alpaca anti-mouse IgG3, Fab-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of a Nanobody that binds to mouse IgG3 with high affinity & specificity.

Reactivity

Mouse

Applications

IF, WB

Conjugate

Alexa Fluor® 647

Cat no : sms3AF647-1

Synonyms

mouse IgG3; mouse immunoglobulin Gamma 3

Validation Data Gallery

  • Anti-mouse IgG3 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG3 antibody (grey) with 2 copies of a monoclonal mouse Fab- specific Nanobody (green) bound. In total, 6 fluorophores (red stars) label the mouse IgG3 primary antibody.

    Anti-mouse IgG3 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG3 antibody (grey) with 2 copies of a monoclonal mouse Fab- specific Nanobody (green) bound. In total, 6 fluorophores (red stars) label the mouse IgG3 primary antibody.

  • HeLa cells were immunostained with mouse IgG3 anti-Lamin A/C antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor? 647 (magenta). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    HeLa cells were immunostained with mouse IgG3 anti-Lamin A/C antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor? 647 (magenta). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • One-step staining (left) vs. sequential staining (right) of HeLa cells with anti-MOT (mitochondria) mouse IgG3 monoclonal primary antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor? 647 (magenta). Cell nuclei are stained with DAPI (blue). Scale bar, 20 μm.

    One-step staining (left) vs. sequential staining (right) of HeLa cells with anti-MOT (mitochondria) mouse IgG3 monoclonal primary antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor? 647 (magenta). Cell nuclei are stained with DAPI (blue). Scale bar, 20 μm.

  • Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one conventional secondary antibody. Green: mouse IgG3 anti-Lamin + alpaca anti-mouse IgG3 VHH Alexa Fluor? 488. Red: mouse IgG1 anti-Tubulin + alpaca anti-mouse gG1 VHH Alexa Fluor? 568. Magenta: rabbit anti-Ki67 + conventional polyclonal secondary anti-rabbit-AF647. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one conventional secondary antibody. Green: mouse IgG3 anti-Lamin + alpaca anti-mouse IgG3 VHH Alexa Fluor? 488. Red: mouse IgG1 anti-Tubulin + alpaca anti-mouse gG1 VHH Alexa Fluor? 568. Magenta: rabbit anti-Ki67 + conventional polyclonal secondary anti-rabbit-AF647. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Higher resolution with anti-mouse IgG3 Nano-Secondary compared to conventional secondary antibodies. Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary antibody.

    Higher resolution with anti-mouse IgG3 Nano-Secondary compared to conventional secondary antibodies. Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary antibody.

Product Information

Nano-Secondary® alpaca anti-mouse IgG3, Fab-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of a Nanobody that binds to mouse IgG3 with high affinity & specificity.

ApplicationsIF, WB
Recommended DilutionImmunofluorescence 1:1,000
Western blot 1:1,000
Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000.
Note: Image acquisition time may have to be optimized.
ClonalityMonoclonal
Host
AffinityCTK0107: KD = 5.7 nM
Specificity/TargetMouse IgG3; this Nanobody recognizes the Fab fragment of mouse IgG3
ReactivityMouse
Conjugate0.5 g/ L
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionStore at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.