Alpha SNAP Monoclonal antibody

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  WB analysis using 67323-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67323-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 67323-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67323-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67323-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 67323-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Alpha SNAP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67323-1-Ig

Clone No.1E2A2

Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

Alpha SNAP, NAPA, SNAP alpha, SNAPA

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Tested Applications

• Positive WB detected in: pig brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67323-1-Ig targets Alpha SNAP in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13180

  Product name: Recombinant human NAPA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-295 aa of BC007432

  Sequence: MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR

• Full Name: N-ethylmaleimide-sensitive factor attachment protein, alpha
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC007432
• Gene Symbol: Alpha SNAP
• Gene ID (NCBI): 8775
• RRID: AB_2882583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54920
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Sci Rep; αSnap plays a pivotal role in the maintenance of Drosophila ISCs survival and tissue homeostasis; Authors - Ying Chen