c-SRC Polyclonal antibody
c-SRC Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Cat no : 25978-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, CHO cells, COLO 320 cells, Jurkat cells, mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25978-1-AP is c-SRC Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||c-SRC fusion protein Ag22415|
|Full Name||v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC011566|
|Gene ID (NCBI)||6714|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.
|Product Specific Protocols|
|WB protocol for c-SRC antibody 25978-1-AP||Download protocol|
|IHC protocol for c-SRC antibody 25978-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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