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ChromoTek RFP-Trap® Dynabeads

RFP-Trap® Dynabeads for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Dynabeads™.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP

Conjugate

Dynabeads

Cat no : rtd

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

Validation Data Gallery

  • Immunoprecipitation (IP) of mCherry with RFP-Trap Dynabeads. I: Input, FT: Flow-Through, B: Bound

    Immunoprecipitation (IP) of mCherry with RFP-Trap Dynabeads. I: Input, FT: Flow-Through, B: Bound

  • Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Dynabeads. I: Input, FT: Flow-Through, B: Bound

    Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Dynabeads. I: Input, FT: Flow-Through, B: Bound

  • Cartoon to visualize the binding of large protein with a protein tag (tag (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (tag (green) + protein of interest (POI, blue) to Nanobody (dark green) of Nano-Trap Magnetic Agarose or Nano-Trap Dynabeads.

    Cartoon to visualize the binding of large protein with a protein tag (tag (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (tag (green) + protein of interest (POI, blue) to Nanobody (dark green) of Nano-Trap Magnetic Agarose or Nano-Trap Dynabeads.

Product Information

RFP-Trap® Dynabeads for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Dynabeads™.

ApplicationsIP, CoIP
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateDynabeads
Beads~ 2.8 µm (Magnetic, high throughput-compatible)
Binding capacity10 µL slurry bind ~ 0.5 µg of recombinant mCherry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage Condition Upon receipt store at +4°C. Do not freeze!