ChromoTek RFP-Trap® Dynabeads
RFP-Trap® Dynabeads for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Dynabeads™.
Ready to use reagent
Specificity
mCherry, mRFP, mPlum
Applications
IP, CoIP
Conjugate
Dynabeads
Cat no : rtd
Synonyms
Validation Data Gallery
Product Information
RFP-Trap® Dynabeads for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Dynabeads™.
Applications | IP, CoIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 5 nM |
Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
Coupled VHH | Recombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment |
Conjugate | Dynabeads |
Beads | ~ 2.8 µm (Magnetic, high throughput-compatible) |
Binding capacity | 10 µL slurry bind ~ 0.5 µg of recombinant mCherry |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS RFP-Trap Dynabeads |
Protocol |
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Protocol RFP-Trap Dynabeads?(PDF) |
Brochure |
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RFP-Trap product brochure |
RFP-Trap Specificity |
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Fluorescent protein specificity table |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |