ChromoTek V5-Trap® Magnetic Agarose

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : v5tma

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap



Product Information

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

DescriptionImmunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Binding capacity17.5 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferV5-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 40 nM
Compatibility with mass spectrometryThe V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2868498
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP-MS

Proc Natl Acad Sci U S A

Disproportionate investment in Spiralin B production limits in-host growth and favors the vertical transmission of Spiroplasma insect endosymbionts.

Authors - Florent Masson
IP

J Neurosci

Cnksr2 loss in mice leads to increased neural activity and behavioral phenotypes of Epilepsy-Aphasia Syndrome.

Authors - Eda Erata
IP

Nat Cardiovasc Res

Syndecan-2 selectively regulates VEGF-induced vascular permeability

Authors - F Corti