ChromoTek V5-Trap™ Magnetic Agarose

The ChromoTek V5-Trap™ Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat no : v5tma

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap



Product Information

The ChromoTek V5-Trap™ Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 40 nM
Specificity/TargetV5-tag amino acid sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Coupled VHHrecombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment
ConjugateMagnetic Agarose
Beads~ 40 µm
Binding capacity10 µL slurry bind ~ 7 µg of recombinant V5-tagged protein ~30 kDa
Elution bufferV5-peptide. For details see below.
SDS sample buffer
0.1 M glycine pH 2.0
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.