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ChromoTek Spot-Label ATTO594

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

Specificity

Spot-Tag

Applications

IF, WB

Conjugate

ATTO594

Cat no : eba594

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Label

Validation Data Gallery

  • STED: IF of Spot-tagged Actin-Chromobody with Spot-Label Atto594 bivalent (1:1,000). Gated STED images were acquired with a Leica TCS SP8 STED 3X microscope with pulsed White Light Laser excitation at 590 nm and pulsed depletion with a 775 nm laser. Objective: 100x Oil STED White, NA: 1.4. Pixel size: 21 x 21 nm; z-Step size of z-Stacks: 0.16 µm. Images were deconvolved with Huygens Professional (SVI). STED images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    STED: IF of Spot-tagged Actin-Chromobody with Spot-Label Atto594 bivalent (1:1,000). Gated STED images were acquired with a Leica TCS SP8 STED 3X microscope with pulsed White Light Laser excitation at 590 nm and pulsed depletion with a 775 nm laser. Objective: 100x Oil STED White, NA: 1.4. Pixel size: 21 x 21 nm; z-Step size of z-Stacks: 0.16 µm. Images were deconvolved with Huygens Professional (SVI). STED images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Application of Spot-tag related products.

    Application of Spot-tag related products.

  • Widefield images of HeLa cells expressing Actin-Chromobody-EGFP-Spot-Tag immunostained with Spot-Label ATTO594, bivalent (1:5,000). Left: GFP channel, middle: channel for ATTO594, right: overlay, also with DAPI. Scale bar, 10 µm.

    Widefield images of HeLa cells expressing Actin-Chromobody-EGFP-Spot-Tag immunostained with Spot-Label ATTO594, bivalent (1:5,000). Left: GFP channel, middle: channel for ATTO594, right: overlay, also with DAPI. Scale bar, 10 µm.

  • Confocal images of HeLa cells expressing Tom70-EGFP-Spot-Tag immunostained with Spot-Label ATTO594 bivalent (1:1,000). Left: EGFP fluorescence, middle: ATTO594 signal, right: merge. Scale bar, 10 µm. The images were acquired with Leica TCS SP8 microscope, 100X oil objective, at the Core Facility Bioimaging at the Biomedical Center, LMU Munich, and deconvolved with Huygens Professional (SVI). Left: GFP fluorescence, middle: ATTO594 signal, right: merge. Scale bar, 10 µm.

    Confocal images of HeLa cells expressing Tom70-EGFP-Spot-Tag immunostained with Spot-Label ATTO594 bivalent (1:1,000). Left: EGFP fluorescence, middle: ATTO594 signal, right: merge. Scale bar, 10 µm. The images were acquired with Leica TCS SP8 microscope, 100X oil objective, at the Core Facility Bioimaging at the Biomedical Center, LMU Munich, and deconvolved with Huygens Professional (SVI). Left: GFP fluorescence, middle: ATTO594 signal, right: merge. Scale bar, 10 µm.

Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

ApplicationsIF, WB
Recommended DilutionIF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag (PDRVRAVSHWSS)
ConjugateATTO594
Storage BufferBuffered aqueous solution (PBS)
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C / 40°F. Stable for 6 months. Do not freeze. Protect from light.