Cleaved Caspase 3 Polyclonal antibody

Cleaved Caspase 3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

47

Cat no : 25128-1-AP

Synonyms

active capsase 3, Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Cleaved Caspase 3; P17, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Validation Data Gallery

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  WB analysis using 25128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 25128-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Cleaved Caspase 3 antibody (25128-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 40 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Product Information

25128-1-AP targets Cleaved Caspase 3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, goat, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 17 kDa, 19 kDa
• GenBank Accession Number: NM_032991
• Gene Symbol: CASP3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17, p19 and p32 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 and 19 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publications

Species	Application	Title
human	WB	Small; A Responsive Nanorobot Modulates Intracellular Zinc Homeostasis to Amplify Mitochondria-Targeted Phototherapy; Authors - Xinyuan Zhou
chicken	WB,IF	Sci Total Environ; MitomiR-1736-3p regulates copper-induced mitochondrial pathway apoptosis by inhibiting AATF in chicken hepatocytes; Authors - Yuanxu Li
human,mouse	WB,IHC	Cancer Lett; Icaritin with autophagy/mitophagy inhibitors synergistically enhances anticancer efficacy and apoptotic effects through PINK1/Parkin-mediated mitophagy in hepatocellular carcinoma; Authors - Piao Luo
mouse	IF	ACS Appl Mater Interfaces; Proton-Gradient-Driven Porphyrin-Based Liposome Remote-Loaded with Imiquimod as In Situ Nanoadjuvants for Synergistically Augmented Tumor Photoimmunotherapy; Authors - Dechun Liu
		Elife; The reciprocal regulation between mitochondrial-associated membranes and Notch signaling in skeletal muscle atrophy; Authors - Yurika Ito
human,mouse	WB,IHC	J Transl Med; Cirsiliol induces autophagy and mitochondrial apoptosis through the AKT/FOXO1 axis and influences methotrexate resistance in osteosarcoma; Authors - Mengliang Luo