domain-II-of-FIZ1 Polyclonal antibody

domain-II-of-FIZ1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15827-1-AP

Synonyms

domain I of FIZ 1, domain II of FIZ1, FIZ1, FLT3 interacting zinc finger 1, Zinc finger protein 798, ZNF798

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Validation Data Gallery

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  WB analysis of human kidney using 15827-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15827-1-AP (domain-II-of-FIZ1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15827-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15827-1-AP (domain-II-of-FIZ1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15827-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15827-1-AP (domain-II-of-FIZ1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15827-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15827-1-AP (domain-II-of-FIZ1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15827-1-AP targets domain-II-of-FIZ1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: FLT3-interacting zinc finger 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_032836
• Gene Symbol: FIZ1
• Gene ID (NCBI): 84922
• RRID: AB_2102545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FIZ1, also named as ZNF798, may be a transcriptional repressor of NRL function in photoreceptors. FIZ1 does not repress CRX-mediated transactivation.