|Positive WB detected in||mouse brain tissue, mouse testis tissue, mouse lung tissue, human testis tissue|
|Positive IP detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
10031-2-AP targets Dynamitin (1-83 AA) in WB, IP,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||dynactin 2 (p50)|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC000718|
|Gene ID (NCBI)||10540|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 59aa of full length p50 dynamitin.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
X (Verified Customer) (10-29-2020)
A very good p50 antibody to detect p50 at the righ MW by Western.