Dynamitin (1-83 AA) Polyclonal antibody

Dynamitin (1-83 AA) Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 10031-2-AP

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50



Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, mouse lung tissue, human testis tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10031-2-AP targets Dynamitin (1-83 AA) in WB, IP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name dynactin 2 (p50)
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000718
Gene symbol DCTN2
Gene ID (NCBI) 10540
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 59aa of full length p50 dynamitin.

Protocols

Product Specific Protocols
WB protocol for Dynamitin (1-83 AA) antibody 10031-2-APDownload protocol
IP protocol for Dynamitin (1-83 AA) antibody 10031-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Med

PMID: 19838201

Authors - Cheng Zhiyong Z

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

X (Verified Customer) (10-29-2020)

A very good p50 antibody to detect p50 at the righ MW by Western.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain/human brain lysates
Dynamitin (1-83 AA) Antibody Western Blot validation (1:2000 dilution) in mouse brain/human brain lysates (Cat no:10031-2-AP)