Dynamitin (1-401 AA) Polyclonal antibody

Dynamitin (1-401 AA) Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 11361-1-AP

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, Dynamitin (1-401 AA), p50 dynamitin, RBP50

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Validation Data Gallery

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  WB analysis using 11361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11361-1-AP (Dynamitin (1-401 AA) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 11361-1-AP

  1X10^6 HeLa cells were stained with 1ug Dynamitin antibody (11361-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A375 cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, rat brain tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 1.00 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11361-1-AP targets Dynamitin (1-401 AA) in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Dynamitin (1-401 AA) fusion protein Ag1902
• Full Name: dynactin 2 (p50)
• Calculated Molecular Weight: 401 aa, 44 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC014083
• Gene Symbol: DCTN2
• Gene ID (NCBI): 10540
• RRID: AB_2090609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83aa is responsible for calmodulin binding. This antibody is against the full length p50 dynamitin.