Dynamitin (1-401 AA) Monoclonal antibody

Dynamitin (1-401 AA) Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2B9A5

Cat no : 60257-1-Ig

Synonyms

DCTN2, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, Dynamitin



Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 60257-1-Ig is Dynamitin (1-401 AA) Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Dynamitin (1-401 AA) fusion protein Ag1902
Full Name dynactin 2 (p50)
Calculated molecular weight 401 aa, 44 kDa
Observed molecular weight 52+150 kDa
GenBank accession numberBC014083
Gene symbol DCTN2
Gene ID (NCBI) 10540
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin.

Protocols

Product Specific Protocols
WB protocol for Dynamitin (1-401 AA) antibody 60257-1-IgDownload protocol
IHC protocol for Dynamitin (1-401 AA) antibody 60257-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Dev Biol

Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster.

Authors - Chun-Hong Wu