fetuin-B Polyclonal antibody

fetuin-B Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18052-1-AP

Synonyms

FETUB, 16G2, Gugu, Fetuin-like protein IRL685, FetuinB

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Validation Data Gallery

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  WB analysis of human plasma using 18052-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 18052-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 18052-1-AP

  IP result of anti-fetuin-B (IP:18052-1-AP, 4ug; Detection:18052-1-AP 1:2000) with human plasma lysate 4000 ug.

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  IHC staining of human liver cancer using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human blood tissue, human plasma
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18052-1-AP targets fetuin-B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fetuin-B fusion protein Ag12562
• Full Name: fetuin B
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC074734
• Gene Symbol: Fetuin B
• Gene ID (NCBI): 26998
• RRID: AB_2103181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)