iNOS Recombinant antibody, PBS Only
iNOS Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
6O22
Cat no : 80517-1-PBS
Synonyms
Validation Data Gallery
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
80517-1-PBS targets iNOS in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | nitric oxide synthase 2, inducible |
Calculated Molecular Weight | 131 kDa |
Observed Molecular Weight | 110-130 kDa |
GenBank Accession Number | NM_000625 |
Gene Symbol | NOS2 |
Gene ID (NCBI) | 4843 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS only |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.