iNOS Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80517-1-RR (same clone as 80517-1-PBS)

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)

  LPS treated human PBMCs were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

iNOS Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 80517-1-PBS

Clone No.6O22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

NOS2, Inducible NOS, Inducible NO synthase, HEP-NOS, Hepatocyte NOS

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Product Information

80517-1-PBS targets iNOS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nitric oxide synthase 2, inducible
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: NM_000625
• Gene Symbol: iNOS
• Gene ID (NCBI): 4843
• RRID: AB_2918898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35228
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.