ChromoTek iST Spot-Trap Kit for AP-MS sample preparation of Spot-fusion proteins

Kit for IP/ Co-IP of Spot-tagged proteins & sample preparation for mass spectrometry (MS)

Specificity

Spot-Tag

Applications

IP, MS

Conjugate

Agarose

Cat no : etak-iST

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap



Product Information

Kit for IP/ Co-IP of Spot-tagged proteins & sample preparation for mass spectrometry (MS)

ApplicationsIP, MS
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-tag sequence motif PDRVRAVSHWSS
Coupled VHHRecombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
Spot-Trap Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.