ChromoTek iST V5-Trap™ Kit

Kit for IP/ Co-IP of V5-tagged proteins & sample preparation for mass spectrometry (MS)

Specificity

V5-Tag

Applications

IP, MS

Conjugate

Agarose

Cat no : v5tak-iST

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap



Product Information

Kit for IP/ Co-IP of V5-tagged proteins & sample preparation for mass spectrometry (MS)

ApplicationsIP, MS
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 40 nM
Specificity/TargetV5-tag sequence motif GKPIPNPLLGLDST.
Coupled VHHRecombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 6 µg of recombinant V5-tagged protein (~30 kDa)
Storage Buffer20% ethanol
Storage ConditionKit is shipped at ambient temperature. Upon receipt store V5-Trap at 4°C and Digest at -20°C.

Kit components

ComponentDescription
V5-Trap™ Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.