ChromoTek iST V5-Trap™ Kit

Kit for IP/ Co-IP of V5-tagged proteins & sample preparation for mass spectrometry (MS)

Specificity

V5-Tag

Applications

IP, MS

Conjugate

Agarose

Cat no : v5tak-iST

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap



Product Information

Kit for IP/ Co-IP of V5-tagged proteins & sample preparation for mass spectrometry (MS)

ApplicationsIP, MS
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 40 nM
Specificity/TargetV5-tag sequence motif GKPIPNPLLGLDST.
Coupled VHHRecombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 6 µg of recombinant V5-tagged protein (~30 kDa)
Storage Buffer20% ethanol
Storage ConditionKit is shipped at ambient temperature. Upon receipt store V5-Trap at 4°C and Digest at -20°C.

Kit components

ComponentDescription
V5-Trap™ Agarose 8 reactions plus 2 controls
Digest Enzyme Trypsin-mix to digest proteins.
Resuspend buffer Protease reconstitution buffer for enzymes.
Lyse buffer Denature, reduce and alkylate proteins.
Stop solution Stop the enzymatic activity.
Wash 1 buffer Clean up peptides from hydrophobic contaminants.
Wash 2 buffer Clean up peptides from hydrophilic contaminants.
Elution buffer Elute the peptides from the cartridge.
LC-Load load Load peptides on reversed-phase LC-MS column.
Cartridges Cartridge for 1 to 100 µg protein starting material.
Waste tubes Tube for collecting waste after washing steps.
Collection tubes Tube for collecting peptides after elution.
Adapters Enables placing a cartridge into a tube.
Caps Cap to optionally close the cartridge’s bottom.